chitligsan against pathogenic bacteria (ATCC)
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Chitligsan Against Pathogenic Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 53092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity"
Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity
Journal: RSC Advances
doi: 10.1039/d5ra04969e
Figure Legend Snippet: Characterisation of elemental composition and spectra of Chitligsan. (A) Constituent elements and EDS image patterns, (B) XRF results, and (C) FTIR spectra of Chitligsan.
Techniques Used:
Figure Legend Snippet: Analysis of the XRD patterns of Chitligsan. Calcite was the most abundant mineral (42.8%), followed by hydrotalcite (40.3%), clinopyroxene 15.6%, and ilmenite 1.3%.
Techniques Used:
Figure Legend Snippet: Gas chromatography of volatile compounds generated from pyrolysis of chitosan standard (black trace) and Chitligsan (blue trace).
Techniques Used: Gas Chromatography, Generated
Figure Legend Snippet: Proposed formation mechanism of Chitligsan. Desert sand minerals (Fe 2 O 3 , FeOOH) interact with oxalic acid and light, promoting Fe( iii )/Fe( ii ) redox cycling through chelation and partial reduction under light and moisture, leading to the formation of the mineral composite Chitligsan.
Techniques Used:
Figure Legend Snippet: Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
Techniques Used: Suspension, Control
Figure Legend Snippet: SEM micrographs illustrating the morphological changes of S. aureus (A–C) and E. coli (D–F) upon exposure to Chitligsan or citric acid. (A and D) Untreated control cells; (B and E) cells treated with 0.1 M citric acid; (C and F) cells treated with Chitligsan (1 mg mL −1 ) and 0.1 M citric acid.
Techniques Used: Control
Figure Legend Snippet: MTT assay results for L929 cells after 24 h of exposure to serial dilutions (50 mg mL −1 , 25 mg mL −1 , 12.5 mg mL −1 , 6.25 mg mL −1 ) of Chitligsan stock solution (100 mg mL −1 ). N = 3, * p < 0.05; ** p < 0.01. Control: untreated cells. Positive control (cytotoxic agent): 2.5% DMSO.
Techniques Used: MTT Assay, Control, Positive Control
Figure Legend Snippet: Scratch assay results for L929 cells after 48 h of exposure to Chitligsan (100 mg mL −1 ). Quantification of scratch closure at 24 h and 48 h relative to 0 h in control and Chitligsan-treated groups. The scale bar in the images represents 100 µm. Control: untreated cells. Quantitative analysis of scratch closure (%) at 24 h and 48 h relative to 0 h. (A) Quantification of scratch closure at 24 h and 48 h relative to 0 h, (B) Representative phase-contrast images of scratch wounds at 0 h, 24 h, and 48 h.
Techniques Used: Wound Healing Assay, Control
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